South Africa A will take on India A from Tuesday in the first of three four-day matches at the Mangaung Oval in Bloemfontein.
Spinner Simon Harmer took 10 wickets in the match as the Northerns Titans thrashed Western Province by an innings and 139 runs at Newlands on Sunday.
The only Division One teams to have tasted victory in round one, the Central Gauteng Lions and the EP Warriors, will face off in round two of the 4-Day Series that gets under way on Friday.
The EP Warriors clinched a thrilling one-wicket victory over the Northerns Titans in Centurion on Monday.